Figure 4From: Functional cooperation of of IL-1β and RGS4 in the brachial plexus avulsion mediated brain reorganization Modulation of RGS4 membrane distribution in cultured neuron. Cortical neurons were grown for 10 days, then RGS4 subcellular distribution in the presence or absence of db cAMP, KT5720 (A); IL-1β, IL-1ra (B) was assayed by Western blotting. The localization of PKCβ within lipid rafts microdomain was assayed by discontinuous sucrose centrifugation and Western blotting (C). IL-6 content in the presence or absence of IL-1β, IL-1ra was evaluated by ELISA assay (D). The results were normalized against vehicle treated neurons. Statistical differences were evaluated by ANOVA analysis.*p < 0.05 vs control.Back to article page