Figure 3
From: Localization of SSeCKS in unmyelinated primary sensory neurons
Micrographs demonstrating capsaicin-mediated depletion of a population of primary sensory neurons. (A) FRAP enzyme histochemical reaction product in a section of L4 DRG from a control animal. FRAP reaction product was present within approximately 40% of the small diameter cell bodies. (B) FRAP enzyme histochemical reaction product in a section of L4 DRG from a capsaicin treated animal. The number of somata containing FRAP reaction product was greatly diminished compared to untreated animals (A). (Insets A, B) FRAP enzyme histochemical reaction in transverse sections of L4 spinal cord dorsal horn from control (Inset A) and capsaicin (Inset B) animals. FRAP reaction product was localized within the dorsal third of lamina II of the dorsal horn in control animals and comparatively reduced in capsaicin treated animals. Dorsal is to top. (C) VR-1-immunofluorescence in a section of L4 DRG from a control animal. (D) VR-1-immunofluorescence in a section of L4 DRG from a capsaicin treated animal. The number of somata containing VR-1-immunofluorescence is greatly diminished compared to untreated animals (A). (Insets C, D) VR-1-immunofluorescence in transverse sections of L4 spinal cord dorsal horn from control (Inset C) and capsaicin (Inset D) animals. VR-1 staining was almost completely eliminated following capsaicin treatment.