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Figure 2 | Journal of Brachial Plexus and Peripheral Nerve Injury

Figure 2

From: Stereological analysis of sciatic nerve in chickens following neonatal pinealectomy: an experimental study

Figure 2

The counting principle of the axon number in nerve cross-section. The section of nerve is sampled in a systematic random manner to gain an unbiased estimation of total axon number in a nerve. Each square represents a sampling area. An unbiased counting frame is seen in the center of this area. The axons are counted if nerve fiber being in the unbiased counting frame (f) in each sampling area. Estimates of the total number of myelinated axons are calculated as the product of the number of axons counted in a known fraction and multiplied by the inverse sampling fraction. In this study, upper and right lines of unbiased counting frames represent the inclusion lines (dot lines) and the lower and left lines including the extensions are the exclusion lines. Any profile of myelinated nerve fiber section hitting the exclusion lines is excluded and profile of nerve fiber hitting the inclusion lines and located inside the frame are counted.

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