Modulation of RGS4 membrane distribution in cultured neuron. Cortical neurons were grown for 10 days, then RGS4 subcellular distribution in the presence or absence of db cAMP, KT5720 (A); IL-1β, IL-1ra (B) was assayed by Western blotting. The localization of PKCβ within lipid rafts microdomain was assayed by discontinuous sucrose centrifugation and Western blotting (C). IL-6 content in the presence or absence of IL-1β, IL-1ra was evaluated by ELISA assay (D). The results were normalized against vehicle treated neurons. Statistical differences were evaluated by ANOVA analysis.*p < 0.05 vs control.