Functional cooperation of of IL-1β and RGS4 in the brachial plexus avulsion mediated brain reorganization

Table 1 Genes with confirmed up- or down-regulation

	1 month	3 month	6 month
Regulated in the motor cortex
regulator of G-protein signaling 4 (NM_009062)		↑	↑
protein kinase, cAMP dependent regulatory (NM_008923)		↑	↑
Regulated in the brain stem
IL-1 protein (AJ250429)	↑	↑	↑
interleukin 6 signal transducer (BC058679)	↑	↑	↑
regulator of G-protein signaling 4 (NM_009062)		↑	↑
Regulated in the spinal cord
regulator of G-protein signaling 4 (NM_009062)		↑	↑
protein kinase, cAMP dependent regulatory (NM_008923)		↑	↑

Mice were divided into 4 groups, control, 1, 3 and 6 month of brachial plexus avulsion (n = 5). Cerebral cortex, brainstem and spinal cord were dissected (50 mg, n = 5), mRNA was extracted and Real time PCR analysis was performed with SYBR Green I. Endogenous control (GAPDH) was used for each sample in the same plate, minimizing any effect of plate-to-plate variability. Gene expression was quantified with the 2−ΔΔCt method, which computed the percentage change relative to control.